Selective β2 adrenergic agonist increases Cx43 and miR-451 expression via cAMP-Epac.

نویسندگان

  • Hossein Mostafavi
  • Mojtaba Khaksarian
  • Mohammad Taghi Joghataei
  • Masoud Soleimani
  • Gholamreza Hassanzadeh
  • Sanaz Eftekhari
  • Mansooreh Soleimani
  • Kazem Mousavizadeh
  • Hajar Estiri
  • Sedighesadat Ahmadi
  • Mahmoud Reza Hadjighassem
چکیده

It has been demonstrated that connexin 43 (Cx43) and microRNAs have significant roles in glioma. Cyclic adenosine monophosphate (cAMP) is suggested to be a regulator of connexins and microRNAs. However, it remains elusive whether cAMP and exchange protein directly activated by cAMP (Epac2), have a regulatory effect on Cx43 and microRNA-451 (miR-451) in astrocytoma cells. We treated 1321N1 astrocytoma cells with a selective β2 adrenergic agonist and a selective Epac activator with and without adenyl cyclase and protein kinase A inhibition. Cx43 and miR-451 expression were measured. Next, we evaluated the effect of miR-451 overexpression on Cx43 expression. Cell proliferation was measured using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The results demonstrated that cAMP-Epac2 increased Cx43 and miR-451 expression. However, the alteration of miR-451 expression required a higher dose of drugs. Overexpression of miR-451 had no significant effect on Cx43 expression. The MTT assay showed that cAMP-Epac stimulation and miR-451 overexpression had a synergic inhibitory effect on cell proliferation. These findings may expand our understanding of the molecular biology of glioma and provide new potential therapeutic targets.

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عنوان ژورنال:
  • Molecular medicine reports

دوره 9 6  شماره 

صفحات  -

تاریخ انتشار 2014